Publications

2018

Grainger, S., Willert, K. Mechanisms of Wnt signaling and control. Wiley Interdiscip Rev Syst Biol Med. (2018). DOI: 10.1002/wsbm.1422. PMID: 29600540. PubMed

Grainger, S., Traver, D., Willert, K. Wnt Signaling in Hematological Malignancies.  Prog Mol Biol Transl Sci. (2018) DOI: 10.1016/bs.pmbts.2017.11.002. PubMed

Richter, J., Stanley, E.G., Ng, E.S., Elefanty, A.G., Traver, D., Willert, K. WNT9A Is a Conserved Regulator of Hematopoietic Stem and Progenitor Cell Development. Genes (Basel) (2018). DOI: 10.3390/genes9020066. PubMed

2017

Huggins, I., Bos, T., Gaylord, O., Jessen, C., Lonquich, B., Puranen, A., Richter, J., Rossdam, C., Brafman, D., Gaasterland, T., Willert, K. The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells. Nature Communications (2017). DOI:10.1038/s41467-017-01203-1. PubMed

Brafman, D. and Willert, K. Wnt/β-catenin signaling during early vertebrate neural development. Developmental Neurobiology (2017). DOI:10.1002/dneu.22517. PubMed

Richter, J., Traver, D., Willert, K. The role of Wnt signaling in hematopoietic stem cell development. Critical Reviews in Biochemistry and Molecular Biology, 52(4): 414-424 (2017). PubMed

2016

Grainger, S., Lonquich, B., Oon, C.H., Nguyen, N., Willert, K., Traver, D. CRISPR guide RNA validation in vitro. Zebrafish, DOI: 10.1089/zeb.2016.1358 (2016). PubMed

Grainger, S.*, Richter, J.*, Espín Palazón, R., Pouget, C., Lonquich, B., Wirth, S., Grassme, K. S., Herzog, W., Swift, M. R., Weinstein, B. M., Traver, D., Willert, K. Wnt9a is required for the aortic amplification of nascent hematopoietic stem cells. Cell Reports, 17(6):1595-1606 (2016). PubMed

Belinson, H., Nakaatani, J., Babineau, B., Birnbaum, R., Ellegood, J., Bershteyn, M., McEvilly, R., Long, J., Willert, K., Klein, O., Ahituv, N., Lerch, J., Rosenfeld, M., Wynshaw-Boris, A. Prenatal β-catenin/Brn2/Tbr2 transcriptional cascade regulates adult social and stereotypic behaviors. Molecular Psychiatry, 21(10):1417-33 (2016). PubMed

 

2011-2015

Kumar, N., Richter, J., Cutts, J., Bush, K.T., Trujillo, C., Nigam, S.K., Gaasterland, T., Brafman, D., Willert, K. Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. ELife, 4:e08413. DOI: 10.7554/eLife.08413 (2015). PubMed

Aznar, N., Midde, K., Dunkel, Y., Lopez-Sanchez, I., Pavlova, Y., Marivin, A., Barbazán, J., Murray, F., Nitsche, U., Janssen, K.-P., Willert, K., Goel, A., Abal, M., Garcia-Marcos, M., Ghosh, P. Daple is a Novel Non-Receptor GEF Required for Trimeric G protein Activation in Wnt Signaling. ELife, 4:e07091 (2015). PubMed

Ross, J., Busch, J., Mintz, E., Ng, D., Stanley, A., Brafman, D., Sutton, V.R., Van den Veyver, I., Willert, K. A rare human syndrome provides genetic evidence that WNT signaling is required for reprogramming of fibroblasts into induced pluripotent stem cells. Cell Reports, 9:1770-80 (2014). PubMed

Moya, N., Gaasterland, T., Willert, K., Brafman, D. Endogenous WNT signaling regulates hESC-derived neural progenitor cell heterogeneity and specifies their regional identity. Stem Cell Reports, 3:1015-28 (2014). PubMed

Fernandez, A., Huggins, I. J., Perna, L., Brafman, D., Lu, D., Yao, S., Gaasterland, T., Carson, D., Willert K. The WNT receptor FZD7 is required for maintenance of the pluripotent state in human embryonic stem cells. Proc Natl Acad Sci U S A, 111:1409-14 (2014). PubMed

Bauer, M., Bénard, J., Gaasterland, T., Willert, K., Cappellen, D. WNT5A encodes two isoforms with distinct functions in cancers. PLoS ONE, 8:e80526 (2013). PubMed

Brafman, D., Moya, N., Soltero, S., Fellner, T., Robinson, M., Vomberg-McMillen, Z., Gaasterland, T., Willert, K. Analysis of SOX2 expressing cell populations derived from human pluripotent stem cells. Stem Cell Reports, 1: 464-78 (2013). PubMed

Green, J.L., Bauer, M., Yum, K.W., Li, Y.C., Cox, M.L., Willert, K., Wahl, G.M. Use of a molecular genetic platform technology to produce human Wnt proteins reveals distinct local and distal signaling activities. PLoS One, 8:e58395 (2013). PubMed

Brafman, D., Phung, C., Kumar, N., Willert, K. Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions. Cell Death and Differentiation, 20:369-81 (2013). PubMed

Brafman, D., Chien, S., Willert, K.  Arrayed cellular microenvironments for identifying culture and differentiation conditions for stem, primary and rare cell populations. Nature Protocols 7, 703-17 (2012). PubMed

Bauer, M., Willert, K. Wnt Signaling: The β-cat(enin)’s Meow. Genes and Development 26, 105-9 (2012). PubMed

Gallegos, T.F., Kouznetsova, V., Kudlicka, K., Sweeney, D.E., Bush, K.T., Willert, K., Farquhar, M.G., Nigam, S.K. A Protein Kinase A and Wnt-dependent network regulating an intermediate stage in epithelial tubulogenesis during kidney development. Developmental Biology, 364, 11-21 (2012). PubMed

Mulligan, K., Fuerer, C., Ching, W., Fish, M., Willert, K., Nusse, R. Secreted Wingless-interacting molecule (SWIM) promotes long-range signaling by maintaining Wingless solubility. Proc Natl Acad Sci U S A, 109:370-7 (2011) PubMed

 

2006-2010

Brafman, D., Chang, C.-W., Fernandez, A., Willert, K., Varghese, S., Chien, S. Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces. Biomaterials, 31, 9135-9144 (2010). PubMed

Hu, T., Li, C., Cao, Z., Van Raay, T.J., Smith, J.G., Willert, K., Solnica-Krezel, L., Coffey, R.J. Myristoylated naked2 antagonizes WNT-β-catenin activity by degrading dishevelled-1 at the plasma membrane. J. Biol. Chem., 285(18):13561-8 (2010) PubMed

Lu, D., Liu, J.X., Endo, T., Zhou, H., Yao, S., Willert, K., Schmidt-Wolf, I.G., Kipps, T.J., Carson, D.A. Ethacrynic acid exhibits selective toxicity to chronic lymphocytic leukemia cells by inhibition of the Wnt/β-catenin pathway. PLoS One, 4(12):e8294 (2009). PubMed

Brafman, D., de Minicis, S., Seki, E., Shah, K.D., Teng, D., Brenner, D., Willert, K., Chien, S. Investigating the Role of the Extracellular Environment in Modulating Hepatic Stellate Cell Biology with Arrayed Combinatorial Microenvironments. Integrative Biology, 1, 513 – 524 (2009). PubMed

Lancaster, M.A., Louie, C.M., Silhavy, J.L., Sintasath, L., DeCambre, M., Nigam, S.K., Willert, K., Gleeson, J.G. Impaired Wnt/β-catenin signaling disrupts adult renal homeostasis and leads to cystic kidney ciliopathy. Nat Med. 15:1046-54 (2009). PubMed

Eguchi, A., Meade, B.R., Chang, Y.-C., Fredrickson, C.T., Willert, K., Puri, N., Dowdy, S.F. Efficient siRNA Delivery into Primary Cells by Peptide Transduction-dsRNA Binding Domain (PTD-DRBD) Fusion Protein. Nat. Biotechnology, 27:567-71 (2009). PubMed

Brafman, D.A., Shah, K.D., Fellner, T., Chien, S., Willert, K. Defining long-term maintenance conditions of human embryonic stem cells with arrayed cellular microenvironment technology. Stem Cells Dev. 18:1141-54. (2009). PubMed

Soda M., Willert K., Kaushansky K., Geddis, A. Inhibition of GSK-3 promotes survival and proliferation of megakaryocytic cells through -catenin-independent signaling. Cellular Signaling. 20:2317-23 (2008). PubMed

Willert K., Jones, K. Wnt signaling: Is the party in the nucleus? Genes and Development 20, 1394-1404 (2006). PubMed

Galli L.M., Barnes T., Cheng T., Acosta L., Anglade A., Willert K., Nusse R., Burrus L.W. Differential Inhibition of Wnt-3a by Sfrp-1, Sfrp-2, and Sfrp-3. Developmental Dynamics 235, 681-90 (2006). PubMed

 

2001-2005

Duncan, A.W., Rattis, F.M., DiMascio, L.N., Congdon, K.L., Paxianos, G, Zhao, C., Yoon, K., Cook, J. M., Willert, K., Gaiano, N., Reya, T.  Integration of Notch and Wnt signaling in hematopoietic stem cell maintenance.  Nature Immunology. 6, 448-52 (2005). PubMed

Galli L.M., Willert K., Nusse R., Yablonka-Reuveni Z., Nohno T., Denetclaw W., Burrus L.W. A proliferative role for Wnt-3a in chick somites. Dev Biol. 269, 489-504 (2004). PubMed

Willert, K., Brown J., Danenberg E., Duncan A., Weissman I.L., Reya T., Yates III J.R., and Nusse R. Wnt proteins are lipid-modified and can act as stem cell factors. Nature 423, 448-452 (2003). PubMed

Reya, T., Duncan A., Ailles L., Domen J., Scherer D., Willert K., Hintz L., Nusse R., and Weissman I.L. A role for Wnt signalling in self-renewal of haematopoietic stem cells. Nature 423, 409-414 (2003). PubMed

Wilson, S., Rydström, A., Trimborn, T., Willert, K., Nusse, R., Jessel, T., and Edlund, T. The status of Wnt signalling regulates neural and epidermal fates in the chick embryo. Nature 411, 325-330 (2001) PubMed

2000 and before

Reya, T., O’Riordan, M., Okamura, R., Devaney, E., Willert, K., Nusse, R., and Grosschedl, R. Wnt signaling regulates B lymphocyte proliferation through a LEF-1 dependent mechanism. Immunity 13, 15-24, (2000). PubMed

Willert, K., Logan, C.Y., Arora, A., Fish, M., and Nusse, R. A Drosophila Axin homolog, Daxin, inhibits Wnt signaling. Development 126, 4165-4173 (1999). PubMed

Willert, K., Shibamoto, S., and Nusse, R. Wnt-induced dephosphorylation of Axin releases b-catenin from the Axin complex.  Genes and Development 13, 1768-1773 (1999). PubMed

Millar, S. E., Willert, K., Salinas, P. C., Roelink, H., Nusse, R., Sussman, D. J. and Barsh, G. S. WNT signaling in the control of hair growth and structure. Dev Biol 207, 133-149 (1999). PubMed

Willert, K. and Nusse, R. Beta-catenin: a key mediator of Wnt signaling. Curr Opin Genet Dev 8, 95-102 (1998). PubMed

Korinek, V., Barker, N., Willert, K., Molenaar, M., Roose, J., Wagenaar, G., Markman, M., Lamers, W., Destree, O. and Clevers, H. Two members of the Tcf family implicated in Wnt/b-catenin signaling during embryogenesis in the mouse. Mol Cell Biol 18, 1248-1256 (1998). PubMed

Nusse, R., Samos, C. H., Brink, M., Willert, K., Cadigan, K. M., Wodarz, A., Fish, M. and Rulifson, E. Cell culture and whole animal approaches to understanding signaling by Wnt proteins in Drosophila. Cold Spring Harb Symp Quant Biol 62, 185-190 (1997). PubMed

Willert, K., Brink, M., Wodarz, A., Varmus, H. and Nusse, R. Casein kinase 2 associates with and phosphorylates dishevelled. Embo J 16, 3089-3096 (1997). PubMed

Shackleford, G. M., Willert, K., Wang, J. and Varmus, H. E. The Wnt-1 proto-oncogene induces changes in morphology, gene expression, and growth factor responsiveness in PC12 cells. Neuron 11, 865-875 (1993). PubMed

Young, J. A., Bates, P., Willert, K. and Varmus, H. E. Efficient incorporation of human CD4 protein into avian leukosis virus particles. Science 250, 1421-1423 (1990). PubMed